Metabolomics & Lipidomics Facility
The Cancer Center Shared Resource for Metabolomics & Lipidomics at the University of Virginia is an analytical facility specializing in mass spectrometry techniques for assessing small molecules. These approaches encompass a variety of targeted, untargeted, qualitative, and quantitative analyses to support investigators in their research. The core has experience with a variety of sample types, including mammalian (cells, tissues, fluids), plants, insects, and biomaterials. Example analyses include the following:
Untargeted metabolite/lipid analyses
Generally a hypothesis-generating experiment, these approaches will profile changes in metabolites between groups.
Targeted metabolite/lipid analyses
Various methods are available for targeted analyses of metabolites to determine steady-state levels. These include amino acids, organic acids, short-chain fatty acids, eicosanoids, acyl-CoAs, acyl-carnitines, sphingolipids, and more. Assays can be developed for your compound(s) of interest.
Flux analyses serve to test mechanisms for altered steady-state levels of metabolites. Stable isotope labeled compounds (e.g. 13C, 2H and/or 15N) are used to assess the activity through a pathway.
Drug Metabolism and Pharmacokinetics
The Core can aid in determining various in vitro and in vivo properties of drugs. Examples include, microsomal stability, half-life, protein binding, and determination of phase I and II metabolites.
Mass spectrometry has applicability beyond the above listed categories. We are open to applying new approaches and developing new methods to aid investigators in their research needs.
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